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Which of the following is NOT a function of the mRNA cap?


A) protects the mRNA from degradation
B) enhances translatability of the mRNA
C) enhances transport of the mRNA to the cytoplasm
D) enhances splicing of the mRNA
E) helps regulate expression of the mRNA

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E

Pre-mRNA processing begins during transcription.

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The addition of poly(A)to mRNA seems to be important for its translatability.

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Which of the following regions on calf-thymus poly(A) polymerase has been shown experimentally not to be necessary for its activity in vitro?


A) RNA binding domain
B) polymerase module
C) nuclear localization signals
D) serine/threonine rich regions
E) All domains are necessary.

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The poly(A) tail of eukaryotic mRNA is approximately ___________ nucleotides in length.


A) 5
B) 25
C) 100
D) 250
E) 1000

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Which of the following is not required for mammalian cell polyadenylation of pre-mRNA?


A) PAP
B) CPSF
C) PABII
D) RNA polymerase I
E) RNA polymerase II

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Once a mRNA loses its poly(A)tail,it is imported back into the nucleus.

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Experiments using yeast as a model have shown that cleavage of the poly(A)tail is necessary for transcription termination.

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The 5' cap of mRNA helps protect the mature mRNA from degradation by ________.

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Which of the following is the mRNA polyadenylation signal motif found in mammalian cells?


A) AAGAAA
B) AAUAAA
C) AAUGGG
D) UUAUUU
E) GGAUUU

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RNA polymerase II transcription of a gene usually stops at the polyadenylation site.

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Poly(A) polymerase can be found in the _____________ of a cell.


A) nucleus
B) cytoplasm
C) lysosomes
D) nucleus and cytoplasm
E) nucleus and lysosomes

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The function of a nucleotide phosphohydrolase is to ______________ phosphates from its substrate.

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Pre-mRNA must be cleaved before it is polyadenylated.

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When does capping of the pre-mRNA occur?


A) It is the first nucleotide added by RNA polymerase.
B) It occurs before the mRNA reaches a chain length of 30 nt.
C) It occurs after the poly(A) tail has been added.
D) It occurs after splicing has occurred.
E) It occurs after export of the mRNA into the cytoplasm.

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At least 5 different cDNAs have been isolated for the enzyme poly(A)polymerase.These cDNAs are a result of alternative ___________ and ______________ of the poly(A)polymerase gene.

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splicing,p...

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___________ RNA is the only type of RNA to get polyadenylated.

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Most genes do not encode for a poly(A)tail.

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The cap plays a role in the removal of the ___________ intron while poly(A)tail plays a role in the removal of the ____________ intron.

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first,last

The capping substance for eukaryotic mRNAs is ______________.

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7-methylguanosine

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