Exam 9: Biotechnology and Recombinant Dna

A) allows the use of bacteria as production factories for a number of molecules.
B) relies on recombinant DNA technology.
C) is dependent on RNA enzymes.
D) relies completely on conjugation.
E) allows the use of bacteria as production factories for a number of molecules AND relies on recombinant DNA technology.

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A) they cut at defined sites.
B) the sticky ends make it very easy to allow recombination of any type of DNA.
C) they protect eukaryotes against virus attack.
D) they cut RNA molecules.
E) they cut at defined sites AND the sticky ends make it very easy to allow recombination of any type of DNA.

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A) density.
B) shape.
C) size.
D) sequence.

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C

A) is always donated to the offspring from both parents
B) can be used to identify related people
C) can be isolated only from intact embryos
D) can be used to establish paternity

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B

A) viruses.
B) bacteria.
C) plant cells.
D) animal cells.

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A) DNAse.
B) DNA ligase.
C) ligandase.
D) polymerase.
E) DNAse AND ligandase.

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A) may use many DNA fragments that function like probes.
B) attaches nucleotides to a solid support such as a glass slide.
C) relies on arrays that contain a detectable tag.
D) uses nucleic acid hybridization.
E) may use many DNA fragments that function like probes, attaches nucleotides to a solid support such as a glass slide AND uses nucleic acid hybridization.

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True

A) detecting viable yet non-culturable organisms.
B) assessing impure (multiple types of bacteria present) samples.
C) dealing with very small numbers of bacteria.
D) relatively quickly producing results.
E) All of the choices are correct.

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A) pest resistant plants.
B) plants that are herbicide resistant.
C) plants with increased nutritional value.
D) All of the choices are correct.

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A) BamHI to cut both sides-since it cuts asymmetrically, it'll leave the sticky, cohesive single-strand DNA ends that will make it easier to ligate into a BamHI-cut plasmid DNA sequence.
B) AluI to cut both sides-it's always easier to ligate together blunt ends of DNA.She should also use AluI on the plasmid she wants to put the fragment into.
C) BamHI on the fragment, and AluI on the plasmid-this will give her the matching sequences to anneal/ligate together on the fragment/plasmid combination.
D) BamHI on one side of the fragment, and AluI on the other side-this would keep the fragment from sticking right back to where it was cut out from in the original DNA.

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A) nigrosin.
B) malachite green.
C) gold oxide.
D) ethidium bromide.

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A) a protein.
B) many copies of the gene to be used as a probe.
C) many copies of the gene for sequencing.
D) more copies of the host cell.
E) a protein, many copies of the gene to be used as a probe AND many copies of the gene for sequencing.

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A) how many cycles are performed.
B) the size of the template DNA.
C) the location to which the primers anneal.
D) how much Taq is used.

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A) Nothing-it's not necessary to wash off the unbound probe.
B) You would get false positive results in different areas where the probe hadn't actually bound, but it was still sitting there and lighting up.
C) Your FISH would be floating at the top of the tank due to the toxicity of the probe building up within them.
D) Nothing-the target nucleotide sequences are labeled, not the probe.Therefore, excess unbound probe wouldn't matter for the experiment.

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A) identifying organisms that have taken up recombinant DNA.
B) searching for RNA.
C) production of proteins.
D) production of DNA.

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