Question 1

Restriction endonucleases recognize and clip at DNA base sequences called _____.

Accepted Answer

palindromes

Question 2

Vectors often contain a gene conferring drug resistance to their cloning host, in order to detect cells harboring the plid.

Accepted Answer

A) True 
 B)False  True

Question 3

All of the following are desirable features in a microbial cloning host, except

Accepted Answer

A)   the host can be grown in large quantities.
B)   the host has a slow growth rate.
C)   the host is capable of accepting plid vectors.
D)   the host will secrete a high yield of proteins from expressed foreign genes.
E)   the host has a well-delineated genome.

Question 4

When determining the sequence of nucleotides in an unknown sample of DNA, which method is used to sequence the DNA?

Accepted Answer

A)   PCR
B)   Cloning
C)   The Sanger Method
D)   Southern blot hybridization
E)   Microarray analysis

Question 5

Which step involves transformation?

Accepted Answer

A)   Target DNA is removed from cells and isolated.
B)   The cloning host takes up a plid.
C)   Target DNA and plid are treated with the same restriction endonuclease.
D)   The desired protein is produced by the cloning host.
E)   The gene is amplified by multiplication of the cloning host.

Question 6

Sequences of DNA that are identical when read from the 5'to 3'direction on one strand and the 3'to 5' direction on the other strand are

Accepted Answer

A)   palindromes.
B)   reverse transcriptases.
C)   restriction endonucleases.
D)   ligases.
E)   DNA polymerases.

Question 7

To be of value, DNA sequences targeted for analysis for DNA profiling need to be located in coding regions of the genome.

Accepted Answer

A) True 
 B)False

Question 8

Which of the following drugs is/are produced by genetic engineering and approved for human use?

Accepted Answer

A)   Human growth hormone
B)   Factor VIII
C)   insulin
D)   tissue plinogen activator
E)   All of these choices are correct.

Question 9

The commercial product Frostban contains a genetically engineered strain of

Accepted Answer

A)   Escherichia coli.
B)   Saccharomyces cerevisiae.
C)   Thermus aquaticus.
D)   Pseudomonas syringae.
E)   Pseudomonas fluorescens.

Question 10

Which of the following would be of LEAST value in DNA profiling?

Accepted Answer

A)   mitotyping
B)   STR short tandem repeat)  analysis
C)   sequencing blood group alleles
D)   analysis of SNPs single nucleotide polymorphisms)

Question 11

Which technique will hopefully be used to identify and devise treatments for diseases based on the genetic profile of the disease?

Accepted Answer

A)   PCR
B)   genetic engineering
C)   antisense technology
D)   microarray analysis
E)   DNA sequencing

Question 12

The process of introducing a needed normal gene into human cells is called DNA mapping.

Accepted Answer

A) True 
 B)False

Question 13

All of the following are correct about DNA fingerprinting, except

Accepted Answer

A)   it displays the unique pattern of an individual's DNA fragments.
B)   it can be used to analyze evidence when only minute amounts of DNA are found.
C)   it cannot be used on specimens older than five years.
D)   it is routine to use it as trial evidence in the U.S.
E)   it can be used on hair and saliva.

Question 14

The Southern Blot technique enables detection of specific sequences

Accepted Answer

A)   in DNA.
B)   in RNA.
C)   of amino acids in proteins.
D)   of sugars in polysaccharides.

Question 15

Some genes in bacteria are repressed by antisense RNA that binds to them.

Accepted Answer

A) True 
 B)False

Question 16

Gene probes can be labeled for detection with

Accepted Answer

A)   enzymes.
B)   fluorescent dyes.
C)   radioisotopes.
D)   All of these choices are correct.

Question 17

Draw and label the specific sequence of procedures, reagents, and materials needed to obtain a human protein from
E. coli cells growing in a lab culture.

Accepted Answer

1. Start by growing E. coli cells in a l...

Question 18

The _____ blot method analyzes DNA, while the _____ method analyzes RNA.

Accepted Answer

Southern; Northern

Question 19

When DNA is heated, the two strands will separate.

Accepted Answer

A) True 
 B)False

Question 20

In order to ensure compatibility between the target DNA and the plid DNA,

Accepted Answer

A)   target DNA is removed from cells and isolated.
B)   cloning host is treated with calcium chloride and receives plid.
C)   target DNA and plid are treated with the same restriction endonuclease.
D)   desired protein is produced by cloning host.
E)   gene is amplified by multiplication of cloning host.

Exam 10: Genetic Engineering: a Revolution in Molecular Biology

Restriction endonucleases recognize and clip at DNA base sequences called _____.

Correct Answerverifiedpalindromes

Vectors often contain a gene conferring drug resistance to their cloning host, in order to detect cells harboring the plid.

Correct AnswerverifiedTrue

All of the following are desirable features in a microbial cloning host, except

Correct AnswerverifiedShow Answer

When determining the sequence of nucleotides in an unknown sample of DNA, which method is used to sequence the DNA?

Correct AnswerverifiedShow Answer

Which step involves transformation?

Correct AnswerverifiedShow Answer

Sequences of DNA that are identical when read from the 5'to 3'direction on one strand and the 3'to 5' direction on the other strand are

Correct AnswerverifiedShow Answer

To be of value, DNA sequences targeted for analysis for DNA profiling need to be located in coding regions of the genome.

Correct AnswerverifiedShow Answer

Which of the following drugs is/are produced by genetic engineering and approved for human use?

Correct AnswerverifiedShow Answer

The commercial product Frostban contains a genetically engineered strain of

Correct AnswerverifiedShow Answer

Which of the following would be of LEAST value in DNA profiling?

Correct AnswerverifiedShow Answer

Which technique will hopefully be used to identify and devise treatments for diseases based on the genetic profile of the disease?

Correct AnswerverifiedShow Answer

The process of introducing a needed normal gene into human cells is called DNA mapping.

Correct AnswerverifiedShow Answer

All of the following are correct about DNA fingerprinting, except

Correct AnswerverifiedShow Answer

The Southern Blot technique enables detection of specific sequences

Correct AnswerverifiedShow Answer

Some genes in bacteria are repressed by antisense RNA that binds to them.

Correct AnswerverifiedShow Answer

Gene probes can be labeled for detection with

Correct AnswerverifiedShow Answer

Draw and label the specific sequence of procedures, reagents, and materials needed to obtain a human protein from E. coli cells growing in a lab culture.

Correct AnswerAnswered by ExamLex AI1. Start by growing E. coli cells in a l...View AnswerShow Answer

The _____ blot method analyzes DNA, while the _____ method analyzes RNA.

Correct AnswerverifiedSouthern; Northern

When DNA is heated, the two strands will separate.

Correct AnswerverifiedShow Answer

In order to ensure compatibility between the target DNA and the plid DNA,

Correct AnswerverifiedShow Answer

Correct Answer
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palindromes

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True

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Correct Answer
Answered by ExamLex AI
1. Start by growing E. coli cells in a l...
View Answer

The _ blot method analyzes DNA, while the _ method analyzes RNA.

Correct Answer
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Southern; Northern

Correct Answer
verified

Correct Answer
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