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Some microbes are not capable of growing on artificial media.

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Which microscope bombards a whole, metal-coated specimen with electrons moving back and forth over it?


A) fluorescence
B) differential interference contrast
C) scanning electron
D) transmission electron
E) phase-contrast

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A microbiologist inoculates Staphylococcus aureus into a culture medium. Following incubation, both Staphylococcus aureus and Staphylococcus epidermidis are determined to be growing in this culture. What is the most likely explanation?


A) The microbiologist used too much inoculum.
B) The culture is contaminated.
C) The incubation temperature was incorrect.
D) The culture medium must be selective.
E) The culture medium must be differential.

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Basic dyes are


A) attracted to the acidic substances of bacterial cells.
B) anionic.
C) used in negative staining.
D) repelled by cells.
E) dyes such as India ink and nigrosin.

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Which of the following will result when 1% to 5% agar is added to nutrient broth, boiled and cooled?


A) a pure culture
B) a mixed culture
C) a solid medium
D) a liquid medium
E) a contaminated medium

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Which is incorrect about chocolate agar?


A) It can be used to cultivate Neisseria.
B) It usually uses sheep blood that has been heated.
C) It has chocolate extract in it.
D) It is an enriched medium.
E) It is used to grow fastidious bacteria.

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The wavelength of light used plus the numerical aperture governs


A) illumination.
B) resolution.
C) magnification.
D) size of the field.
E) All of these choices are correct.

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All of the following are examples of different types of microbiological media except


A) broth.
B) enriched.
C) agar.
D) Petri dish.
E) gelatin.

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Observing and characterizing colonial growth for size, shape, edge, elevation, color, odor, and texture is part of ___.


A) specimen collection
B) inoculation
C) incubation
D) isolation
E) inspection

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Which type of media can be used to determine if a bacteria is motile?


A) SIM
B) MacConkey
C) enriched media
D) Thayer-Martin media
E) chocolate agar

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Sally had a throat sample taken at a satellite lab within her health care provider's office. What kind of media would be best suited for maintaining the quality of this specimen prior to its arrival in the clinical laboratory?


A) TSA plate
B) TSB tube
C) transport media
D) selective agar
E) differential agar

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The bending of light rays as they pass from one medium to another is called refraction.

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A selective medium contains one or more substances that inhibit growth of certain microbes in order to facilitate the growth of other microbes.

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Newly inoculated cultures must be _____ at a specific temperature and time to encourage growth.

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The Gram stain, acid-fast stain, and endospore stain ____________.


A) are used on a wet mount of the specimen
B) use heat to force the dye into cell structures
C) have outcomes based on cell wall differences
D) use a negative stain technique
E) are differential stains

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Magnification is achieved in a compound microscope through the initial magnification of the specimen by the _____ lens. This image is then projected to the _____ lens that will further magnify the specimen to form a virtual image received by the eye.

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A bacterial cell that is deposited on the surface of an appropriate solid growth media will divide repeatedly until it forms a visible accumulation of bacterial growth. This is known as a bacterial


A) colony.
B) community.
C) biofilm.
D) smear.

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If a microbiologist is studying a specimen at a total magnification of 950X, what is the magnifying power of the objective lens if the ocular lens is 10X?


A) 100X
B) 950X
C) 85X
D) 850X
E) 95X

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Mixed cultures are also referred to as contaminated cultures.

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Explain how and why immersion oil increases resolution but not magnification when using the 100X objective.

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Immersion oil increases resolution but n...

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