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Exam 15: Dna and the Gene: Synthesis and Repair

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Question 1

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What can you infer from the information presented in this table?

A) DNA polymerase I and DNA polymerase III have different functions.
B) The sliding clamp molecule is a ribozyme.
C) DNA polymerase I and DNA polymerase III are the same enzyme found in different organisms.
D) Topoisomerase is involved in proofreading activity.

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Question 2

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Figure 15.3 -Refer to Figure 15.3. Which of the structures in the figure breaks hydrogen bonds between complementary bases?

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Question 3

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In the mismatch repair process, enzyme complexes replace bases that were incorrectly inserted into the newly synthesized DNA strand. To function, they must be able to distinguish between the parent DNA strand and the new strand. How is this accomplished?

The epsilon s) subunit of DNA polymerase III of E. coli has exonuclease activity. How does it function in the proofreading process?

\text {Single strand as a template plus 3 ^ { \prime } end to } $\text {start DNA synthesis}$ Figure 15.1 -Refer to Figure 15.1. What bases will be added to the primer as DNA replication proceeds? The bases should appear in the order that they will be added.

If during the process of DNA replication, the enzyme topoisomerase was not functioning i.e. it was mutated) what do you expect will happen to the replication process?

The fact that within a double- stranded DNA molecule, adenine forms two hydrogen bonds with thymine and cytosine forms three hydrogen bonds with guanine is known as

Which of the following agents can cause mutations in DNA?

The transduction experiments done by Hershey and Chase, and the transformation experiments done by Griffith, supported the same conclusion, which was that

What appears to be a dark side to telomerase activity with regard to human health?

Telomere shortening is a problem in which types of cells?

Who performed the classic experiments that proved DNA was copied by semiconservative replication?

How does the simple primary and secondary structure of DNA hold the information needed to code for the many features of multicellular organisms?

Put the following steps of DNA replication in chronological order. 1) Single- stranded binding proteins attach to DNA strands. 2) Hydrogen bonds between base pairs of antiparallel strands are broken. 3) Primase binds to the site of origin. 4) DNA polymerase binds to the template strand. 5) An RNA primer is created.

DNA replication is highly accurate. It results in about one mistake per billion nucleotides. For the human genome, how often would errors occur?

Hereditary nonpolyposis colorectal cancer HNPCC) is an inherited disorder. The genetic defect identified is an error in the mismatch repair mechanism. Which of the following would be an expected result of this mutation?

Semiconservative replication involves a template. What is the template?

Which of the following would you expect of a eukaryote lacking telomerase?

Which one of the following is least likely to cause mutations in DNA?

What are thymine dimers?