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At present, no available method can come close to giving as much information about gene expression as can __________.

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When analyzing the sequence of genes similar to ones already known, why is the amino acid sequence of the protein more important than the DNA sequence?

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Over evolutionary time, a nucleotide seq...

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Explain the technique of two- dimensional (2D) polyacrylamide gel electrophoresis (PAGE).

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A 2D- PAGE separates proteins based on s...

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Genes for DNA replication and transcription make up only a minor fraction of the typical prokaryotic genome.

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An uncharacterized ORF shares with a known protein- encoding gene a(n) _________of reasonable length and __________ and _________codons.

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uninterrupted readin...

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Chromosomal rearrangements due to insertion sequences have apparently contributed to the evolution of several human pathogens.

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Linking an ORF with a specific function is an example of gene


A) discovery.
B) identification.
C) assembly.
D) annotation.

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DNA sequences of_________ prokaryotic genomes are now available in public databases.


A) millions
B) hundreds
C) thousands
D) dozens

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The mitochondrial genomes of almost all animals, including humans, encode


A) rRNA.
B) proteins.
C) tRNA.
D) all of the above.

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Within Archaea and Bacteria, one megabase of DNA encodes about_________ open reading frames.


A) 1,000
B) 100
C) 10,000
D) 10

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The largest cellular genomes belong to prokaryotes that are parasitic or endosymbiotic.

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A functional open reading frame (ORF) encodes for


A) a carbohydrate.
B) DNA or RNA.
C) a protein.
D) all of the above.

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The protozoans Paramecium and Trichomonas have significantly more genes than humans have.

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Comparisons of genes and gene families can be done easily. The results are generally quite predictable, although occasionally there is a surprising find.

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The most abundant genes in prokaryotic genomes are


A) those involved in transport.
B) those involved in translation.
C) those involved in DNA replication.
D) those involved in metabolism.

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Many of the genes in the chloroplast genome encode proteins involved in __________ and_________, whereas mitochondrial genomes primarily encode proteins involved in_________.

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photosynthesis / aut...

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Explain how genomics has shown mitochondrial evolution to be far more complicated than expected.

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Some mitochondria contain accessory plas...

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Explain how evolution can "experiment" with duplicated genes.

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A duplicated gene allows the second gene...

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Explain how codon bias can be used to detect horizontal gene transfer.

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If an organism rarely uses a particular ...

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Explain horizontal gene transfer and discuss how this phenomenon has complicated evolutionary studies.

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Horizontal (or lateral) gene transfer in...

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