Question 1

Allosteric regulation is accomplished by:

Accepted Answer

A)   A small molecule that fits into an enzyme's active site
B)   A large protein that blocks an enzyme's active site
C)   A small molecule that fits into a site on the enzyme that is not the active site
D)   A small molecule that covalently modifies a site on the enzyme that is not the active site

Question 2

If CAP could not bind to its CAP site, then what would be the result? Assume lactose is present in each scenario.

Accepted Answer

A)   Transcription would be difficult to repress in the presence of glucose.
B)   Transcription would be difficult to activate in the presence of glucose
C)   Transcription would be difficult to activate in the absence of glucose
D)   Transcription would be difficult to repress in the presence of glucose

Question 3

If a bacteria is placed in an environment that contains both glucose and lactose, the regulation of the lac operon will allow which nutrient to be processed first?

Accepted Answer

A)   Glucose
B)   Lactose
C)   Both will be processed equally
D)   Neither will be processed in this environment

Question 4

The regulation of protein function, not gene expression is called regulation.

Accepted Answer

A)   Posttranslational
B)   Transcriptional
C)   Translational
D)   Post-transcriptional

Question 5

In the same experiment, what would have been the conclusion if all four tubes produced a yellow color when b-ONPG was added?

Accepted Answer

A)   Expression of the lac operon is constitutive whether lacI is functional or not
B)   LacI provides the binding site for the repressor
C)   LacI encodes a diffusible repressor
D)   The researcher added too much b-ONPG

Question 6

Which cycle would prevail if there were a mutation in the promoter PRE?

Accepted Answer

A)   Lysogenic
B)   Lytic
C)   Neither

Question 7

The regulation of the CAP complex using cAMP is an example of inducible genetic regulation.

Accepted Answer

A) True 
 B)False

Question 8

What stem-loop conformations favor attenuation?

Accepted Answer

A)   1-2
B)   1-2 and 2-3
C)   2-3
D)   1-2 and 3-4

Question 9

Which of the following is not associated with positive transcriptional regulation?

Accepted Answer

A)   Activators
B)   Inducers
C)   Corepressors
D)   All of the answers are involved in positive regulation

Question 10

Repressor proteins are responsible for negative transcriptional regulation.

Accepted Answer

A) True 
 B)False

Question 11

A gene is inducible and under negative control. Which of the following pairs will allow expression of this gene?

Accepted Answer

A)   Activator + repressor
B)   Activator + inhibitor
C)   Repressor + inducer
D)   Repressor + co-repressor

Question 12

Which of the following is found in an operon?

Accepted Answer

A)   Promoter
B)   Terminator
C)   Two or more genes
D)   Operator
E)   All of the answers are correct

Question 13

The term enzyme adaptation is used to describe an enzyme that appears in a living cell following
exposure to a specific substrate.

Accepted Answer

A) True 
 B)False

Question 14

The form of regulation that involves a physical change in the shape of an enzyme is called allosteric
regulation.

Accepted Answer

A) True 
 B)False

Question 15

In the lac operon, the operator site is recognized by a activator protein.

Accepted Answer

A) True 
 B)False

Question 16

A bacterium is transformed with two plasmids, both containing the same gene. In one, the gene is placed next to a strong promoter and in the other, a weak promoter. How would you expect the two transformed bacteria to grow?

Accepted Answer

A)   The bacteria with the strong promoter would grow slower.
B)   The bacteria with the weak promoter would grow slower.
C)   Neither bacteria would grow.
D)   Both bacteria would grow at the same rate.

Exam 14: Gene Regulation in Bacteria and Bacteriophages

A gene is inducible and under negative control. Which of the following pairs will allow expression of this gene?

Correct Answer
verified

A bacterium is transformed with two plasmids, both containing the same gene. In one, the gene is placed next to a strong promoter and in the other, a weak promoter. How would you expect the two transformed bacteria to grow?

Correct Answer
verified

The regulation of protein function, not gene expression is called regulation.

Correct Answer
verified

Which cycle would prevail if there were a mutation in the promoter PRE?

Correct Answer
verified

Which of the following is not associated with positive transcriptional regulation?

Correct Answer
verified

If CAP could not bind to its CAP site, then what would be the result? Assume lactose is present in each scenario.

Correct Answer
verified

What stem-loop conformations favor attenuation?

Correct Answer
verified

The term enzyme adaptation is used to describe an enzyme that appears in a living cell following exposure to a specific substrate.

Correct Answer
verified

In the lac operon, the operator site is recognized by a activator protein.

Correct Answer
verified

The form of regulation that involves a physical change in the shape of an enzyme is called allosteric regulation.

Correct Answer
verified

In the same experiment, what would have been the conclusion if all four tubes produced a yellow color when b-ONPG was added?

Correct Answer
verified

Which of the following is found in an operon?

Correct Answer
verified

Repressor proteins are responsible for negative transcriptional regulation.

Correct Answer
verified

The regulation of the CAP complex using cAMP is an example of inducible genetic regulation.

Correct Answer
verified

Allosteric regulation is accomplished by:

Correct Answer
verified

If a bacteria is placed in an environment that contains both glucose and lactose, the regulation of the lac operon will allow which nutrient to be processed first?

Correct Answer
verified

Exam 14: Gene Regulation in Bacteria and Bacteriophages

A gene is inducible and under negative control. Which of the following pairs will allow expression of this gene?

Correct AnswerverifiedShow Answer

A bacterium is transformed with two plasmids, both containing the same gene. In one, the gene is placed next to a strong promoter and in the other, a weak promoter. How would you expect the two transformed bacteria to grow?

Correct AnswerverifiedShow Answer

The regulation of protein function, not gene expression is called regulation.

Correct AnswerverifiedShow Answer

Which cycle would prevail if there were a mutation in the promoter PRE?

Correct AnswerverifiedShow Answer

Which of the following is not associated with positive transcriptional regulation?

Correct AnswerverifiedShow Answer

If CAP could not bind to its CAP site, then what would be the result? Assume lactose is present in each scenario.

Correct AnswerverifiedShow Answer

What stem-loop conformations favor attenuation?

Correct AnswerverifiedShow Answer

The term enzyme adaptation is used to describe an enzyme that appears in a living cell following exposure to a specific substrate.

Correct AnswerverifiedShow Answer

In the lac operon, the operator site is recognized by a activator protein.

Correct AnswerverifiedShow Answer

The form of regulation that involves a physical change in the shape of an enzyme is called allosteric regulation.

Correct AnswerverifiedShow Answer

In the same experiment, what would have been the conclusion if all four tubes produced a yellow color when b-ONPG was added?

Correct AnswerverifiedShow Answer

Which of the following is found in an operon?

Correct AnswerverifiedShow Answer

Repressor proteins are responsible for negative transcriptional regulation.

Correct AnswerverifiedShow Answer

The regulation of the CAP complex using cAMP is an example of inducible genetic regulation.

Correct AnswerverifiedShow Answer

Allosteric regulation is accomplished by:

Correct AnswerverifiedShow Answer

If a bacteria is placed in an environment that contains both glucose and lactose, the regulation of the lac operon will allow which nutrient to be processed first?

Correct AnswerverifiedShow Answer

Correct Answer
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