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Based on the number of enzymes involved in its biosynthesis, which of the following is the most complex amino acid specified by the genetic code?


A) tryptophan
B) tyrosine
C) phenylalanine
D) leucine
E) isoleucine

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Which molecules use 5-phosphoribosyl-1-pyrophosphate (PRPP) in their biosynthesis?


A) amino acids
B) nucleotides
C) fatty acids
D) tetrapyrrole
E) nonribosomal peptide antibiotics

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It is thought that the Calvin cycle appeared after the divergence of the three domains of life. What supports this hypothesis?

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The full Calvin cycle is only found in b...

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Which of the following is the reducing agent in the saturation step of polyketide biosynthesis?


A) NADH
B) NADPH
C) ferredoxin
D) H2
E) O2

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Briefly describe how NH₄⁺ is assimilated into biomass by bacteria.

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The assimilable ammonium ion (NH₄⁺) is p...

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Which of the following are unique in that they fix CO₂ as their sole carbon source?


A) organotrophs and iron oxidizers
B) photoheterotrophs and chemiolithotrophs
C) photoautotrophs and lithotrophs
D) heterotrophs and methanogens
E) methanogens and photoheterotrophs

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Briefly describe the reductive acetyl-CoA pathway. What is its relevance for methanogens?

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The reductive acetyl-CoA pathway seems to be the most ancient CO₂ fixation and biomass assimilation; for example, in methanogens, up to 5% of the CO₂ they absorb is used in biosynthesis. It consists in the formation of an acetyl group using two CO₂ molecules converging from two different pathways. Formate is produced by reduction of CO₂ and transfer to tetrahydrofolate (THF), thus forming a C1 unit. The C1 unit is reduced to a methyl group, -CH₃. In methanogens, methane is formed from this C1 unit for energy gain. The methyl group can be fixed into biomass after being transferred to a tetrapyrrole (called TB and related to vitamin B₁₂). The second CO₂ molecule becomes \( \mathrm{CO}_{3}^{-} \) via carbon dioxide dehydrogenase \( \mathrm{CO}_{3}^{-} \). is condensed with coenzyme A and the methyl group carried by TB to form acetyl-CoA.

How do ammonium and oxygen regulate the expression of nitrogen fixation genes?

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An operon (nifHDKTY) encodes the nitroge...

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All microorganisms require all of the following for biosynthetic purposes EXCEPT


A) phosphorous.
B) reduction.
C) energy.
D) carbon.
E) nitrogen gas.

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Which of the following statements concerning glyceraldehyde-3-phosphate is FALSE?


A) One G3P is produced by everything three turns of the cycle.
B) Two molecules of G3P condense to form a six-carbon sugar.
C) One more G3P condenses with a sugar to form a larger molecule used for biosynthesis.
D) PGA is reduced to G3P after the PGA moves out of the carboxysome.
E) G3P is reoxidized to carbon dioxide so that the cycle continues.

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E

Chorismate is used in a branched pathway to synthesize


A) fatty acids.
B) polyketide antibiotics
C) purines and pyrimidines
D) aromatic amino acids
E) tetrapyrroles

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Cyanobacteria are believed to generate the majority of oxygen gas in Earth's atmosphere. What allows them to produce oxygen?


A) photosynthesis in which H2S is the electron source
B) photosynthesis in which water photolysis produces H+, e-, and O2
C) bacteriorhodopsin-based photosynthesis
D) being near the surface of a pond where more oxygen is available
E) possession of a variety of chlorophylls to absorb a wide range of wavelengths of light

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Gene expression of nitrogenase through NtrC is downregulated in high concentrations of


A) carbon dioxide.
B) nitrogen.
C) nitrate.
D) ammonium.
E) FeMo protein.

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Certain amino acids are more energetically expensive than others (tryptophan versus glutamate). Thus, when proteins are moved to the cell surface, extended outside the surface, or secreted out of the cell, there is a potential harm. Why is this potentially harmful, and what adaptations do bacteria have for this potential loss? Give an example.

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With more "expensive" amino acids, more ...

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Describe the 3-hydroxypropionate cycle; give examples of microorganisms that use this CO₂ fixation mechanism.

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blured image as blured image condenses with acetyl-CoA to form m...

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Which of the following molecules CANNOT freely cross biological membranes?


A) O2
B) CO2
C) NH4+
D) N2
E) H2

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How is fatty acid biosynthesis regulated in bacteria?

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Because fatty acid biosynthesis consumes...

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The cell diagrammed below provides an overview of some regulation of the Calvin cycle. Which of the following is NOT involved in this regulation?  The cell diagrammed below provides an overview of some regulation of the Calvin cycle. Which of the following is NOT involved in this regulation?   a. Several different transport mechanisms are used for   \mathrm{CO}_{2}   and   \mathrm{HCO}_{3}^{-}-  . b.   \mathrm{CO}_{2}   and   \mathrm{HCO}_{3}^{-}{ }^{-}  are sequestered inside a carboxysome. c. Rubisco is sequestered inside a carboxysome. d. Carbonic anhydrase is used to convert   \mathrm{CO}_{2}   to   \mathrm{HCO}_{3}{ }^{-}  and thus trap it. e. Proteins are used to block light and thus the light reactions. a. Several different transport mechanisms are used for CO2 \mathrm{CO}_{2} and HCO3 \mathrm{HCO}_{3}^{-}- . b. CO2 \mathrm{CO}_{2} and HCO3 \mathrm{HCO}_{3}^{-}{ }^{-} are sequestered inside a carboxysome. c. Rubisco is sequestered inside a carboxysome. d. Carbonic anhydrase is used to convert CO2 \mathrm{CO}_{2} to HCO3 \mathrm{HCO}_{3}{ }^{-} and thus trap it. e. Proteins are used to block light and thus the light reactions.

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In anaplerotic reactions


A) intermediates in a pathway are regenerated.
B) nutrients are imported from the environment.
C) molecules targeted for breakdown are salvaged for anabolism.
D) the reactions are thermodynamically impossible.
E) large amounts of ATP are generated.

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What is the health risk of having nitrates in drinking water? What conditions favor the accumulation of nitrates and nitrites in bodies of water?

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In natural environments most nitrogen sources are subject to competition from dissimilatory metabolism. Fertilizers contain nitrogen as NH₄⁺, which can leach from agricultural soils to bodies of water where it can be oxidized to nitrate and nitrite by lithotrophs, a process known as nitrification. Nitrites can combine with hemoglobin, rendering it incapable of taking up oxygen. When infants drink water with high nitrate content, they become ill with "blue baby" syndrome, as nitrate becomes nitrite in the digestive system.

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