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Exam 6: Dilutions

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Question 1

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Because the tube dilution is the same for all tubes in a serial dilution,the sample dilution for a given tube can be determined using which equation (in all cases N is the number of the tube in the sequence) ?

A) $D_{\text {sample }}=\left(D_{\text {tube }}\right) N$
B) $D_{\text {Sample }}=\left(D_{{tube }}\right) ^{N}$
C) $D_{\text {sample }}=\frac{V_{\text {tube }}}{N}$
D) $D_{\text {sample }}=\frac{N}{V_{\text {tube }}}$

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Question 2

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What is an antibody titer?

A) The amount of antibody bound to a specific antigen in a sample
B) The amount of antibody present in a serum against a particular antigen
C) The concentration of all antibodies present in a sample
D) The volume of serum composed of antibodies

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Question 3

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Which of the following must be done in order to create a standard curve from a single vial of a known concentration of glucose?

Which of the following is a reason to make dilutions?

Which of the following expressions defines the dilution ratio of a sample?

A laboratory technician performs an antibody titer.A reaction is detected in every dilution from 1:2 up to and including 1:64.What does this tell the technician about the amount of antibody present?

A urine sample is diluted 1:3 before testing.The test results identify 50 μg phenylpyruvate.What is the dilution factor that the result must be multiplied by to determine the true concentration of phenylpyruvate in the urine?

How is antibody titer determined?

A 100 μL sample of blood is diluted with 200 μL saline solution.What is the dilution ratio of the sample after dilution?

The initial volume of a sample is 100 μL.It is diluted with saline by a factor of 4.The final volume is 400 μL.Testing the diluted sample finds an analyte concentration of 25 μg/mL.Which of the following expressions can be used to calculate the initial analyte concentration of this sample?

A sample of turbid knee fluid arrives in your laboratory and needs to be analyzed for the presence of white blood cells (WBC) .An initial test reveals that the WBC concentration is higher than the upper limit of the automated blood analyzer.Which of the following should be done in order to achieve an accurate reading?

A laboratory technician is asked to determine the concentration of LDL present in a blood sample.The initial test finds the concentration of LDL to be outside the reliable range for the testing method.Accordingly,she dilutes the blood sample in 100 μL distilled water (dH₂O) .What is the diluent in this scenario?

A laboratory technician is given a blood sample and asked to calculate the concentration of dopamine.The first test reveals that the concentration of dopamine falls outside the reliable range for the testing method.He adds 100 μL saline solution to 100 μL of the sample and retests the sample.How does the concentration of dopamine in the diluted sample compare to the concentration of dopamine in the original sample?

What is a serial dilution?

Which of the following equations defines the tube dilution in a serial dilution?

What does the term "tube dilution" refer to?

A technologist picks up a serum sample with a label indicating it is a 1:4 dilution.What does this tell the technologist about the sample?

When is it advisable to perform a serial dilution rather than a simple dilution?

A sample ready for analysis is a 1:2 dilution.Which of the following is true about this sample?

A urine sample is diluted 1:4 before testing.Testing indicates that the concentration of protein present is 10 μg.What is the true concentration of protein in the urine?