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Based on current research,quorum sensing has only been shown to occur in bioluminescent bacteria.

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The type of regulation that relies on a critical cell density is called ______ _______.

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Which of these is required for transcription that is regulated by positive control?


A) activator protein
B) repressor protein
C) inducer protein
D) attenuator protein
E) DNA polymerase.

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How does lactose regulate the expression of the lac operon?

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The lactose regulation of the lac operon...

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A mutation in the lacI gene results in an active repressor protein that can no longer bind allolactose.Which would be true of the phenotype of the mutant strain?


A) It would be constitutive for β-galactosidase expression.
B) It would overexpress LacZ.
C) It would be the same as the wild-type strain.
D) It would repress β-galactosidase when lactose is present.
E) It would be overexpress β-galactosidase when lactose is present.

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The directed movement toward a light source is called ______________.

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What would characterize the phenotype of a CheY mutant?


A) Loss of motility.
B) Constant tumbling.
C) Smooth swimming.
D) Inability to sense certain chemicals.
E) Movement toward repellents and away from attractants.

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C

Which of these controls the expression of the lac operon?


A) An activator protein only.
B) An attenuation mechanism.
C) A repressor protein only.
D) Both repression and activation mechanisms.
E) Both repression and attenuation mechanisms.

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D

What does sigma factor helps the RNA polymerase do?


A) Assemble tRNA subunits.
B) Bind to activator proteins.
C) Attach the ribosomal subunits.
D) Identify the promoter region of a gene.
E) Release from the transcribed DNA.

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D

During negative regulation of gene expression the repressor protein binds to the operator to inhibit transcription.What is the effector molecule that controls the activity of the repressor protein called?


A) corepressor
B) inducer
C) activator
D) corepressor or inducer
E) corepressor or activator

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Give example of genes that are usually expressed constitutively.Why are these genes constitutively expressed?

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Constitutive gene expression refers to g...

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Isopropyl β-d-1- thiogalactopyranoside (IPTG) ,a lactose analog,acts as an inducer of the lac operon.What is its mode of action?


A) It functions as a co-activator of gene expression.
B) It inactivates the LacI repressor protein.
C) It activates the enzyme β-galactosidase.
D) It binds the RNAP for enhanced binding to the promoter.
E) It binds to the operator to enhance transcription initiation.

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The enzyme β-galactosidase hydrolyzes lactose to glucose and fructose.

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What is quorum sensing? How is it involved in the bioluminescence of the bacterium Vibrio fischeri?

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Quorum sensing is a process used by bact...

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The expression of genes required for biofilm formation occurs only after a cell has attached to a surface that is suitable for biofilm development.What is this type of gene expression called?


A) attenuation
B) feedback inhibition
C) induction
D) repression
E) inhibition

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The two-component regulatory systems uses a _______ ________ and a response regulator.

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What is an effector molecule?


A) A protein that binds to an operator region of a gene.
B) An activator protein for gene expression.
C) A small RNA molecule that inhibits translation of mRNA.
D) A small molecule that binds to an enzyme to regulate its activity.
E) A small molecule that binds to the operator region of a gene.

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The transfer of T-DNA into a plant cell by Agrobacterium tumefaciens is a highly regulated process.This process only occurs if a plant becomes wounded and excretes various compounds that are detected by A.tumefaciens for expression of its virulence genes.Regulation of virulence gene expression is under the control of which type of system?


A) A two-component system.
B) An inducer/repressor system.
C) An activator/co-activator system.
D) A co-repressor/repressor system.
E) A repressor/co-activator system.

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What is the function of methyl-accepting chemotaxis proteins (MCPs) ?


A) To phosphorylate CheY.
B) To control the direction of flagellar rotation.
C) To dephosphorylate CheY.
D) To sense the presence of a specific chemical.
E) To drive the flagellum motors.

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The catabolite activator protein (CAP) activates transcription of the lac operon when it binds this coactivator.


A) lactose
B) glucose
C) cyclic-AMP
D) ATP
E) allolactose

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