Question 1

DNA that contains instructions for two or more structural genes produces monocistronic mRNA.

Accepted Answer

A) True 
 B)False

Question 2

In Jacob, Monod, and Pardee's experiment, how many functional copies of lacI were there in the merozygote?

Accepted Answer

A)   0
B)   1
C)   2
D)   3

Question 3

How many promoters are in an operon?

Accepted Answer

A)  1
B)  2
C)  3
D)  It depends on how many genes are present in the operon

Question 4

An enzyme catalyzes a substrate into a final product. When the concentration of the final product is high enough, it binds to a regulatory site on the enzyme. This binding at the regulatory site changes the shape of the enzyme, which prevents it from binding the substrate and prevents formation of more final product. This is an example of a(n)  _______________.

Accepted Answer

A)  posttranslational modification
B)  allosteric enzyme
C)  translational repressor
D)  methylation

Question 5

Operons that code for catabolic enzyme systems are typically regulated by repressors.

Accepted Answer

A) True 
 B)False

Question 6

A riboswitch only affects translation of an operon.

Accepted Answer

A) True 
 B)False  False

Question 7

In the lac operon, the operator is an example of a trans-effect genetic regulation.

Accepted Answer

A) True 
 B)False

Question 8

Enzymes involved in metabolism are most likely regulated via _____.

Accepted Answer

A)   feedback inhibition
B)   acetylation
C)   methylation
D)   none of the answers are correct

Question 9

The regulation of protein function is called ______.

Accepted Answer

A)  posttranslational regulation
B)  transcriptional regulation
C)  translational regulation
D)  posttranscriptional regulation

Question 10

In the lac operon, the CAP site is located next to the ____________. When both lactose and glucose are present, this leads to a rate of transcription that is ___________.

Accepted Answer

A)  terminator, low
B)  promoter, high
C)  terminator, high
D)  promoter, low

Question 11

In the lac operon, the operator site is recognized by an activator protein.

Accepted Answer

A) True 
 B)False

Question 12

What stem-loop conformations favor attenuation?

Accepted Answer

A)  1-2
B)  1-2 and 2-3
C)  2-3
D)  1-2 and 3-4

Question 13

If CAP could not bind to its CAP site, then what would be the result? Assume lactose is present in each scenario.

Accepted Answer

A)  Transcription would be difficult to repress in the presence of glucose
B)  Transcription would be difficult to activate in the presence of glucose
C)  Transcription would be difficult to activate in the absence of glucose

Question 14

What is the gene responsible for attenuation in the trp operon?

Accepted Answer

A)  trpL
B)  trpR
C)  trpD
D)  trpC

Question 15

A deletion in an operon removes the terminator. How will that affect the transcript that is produced from the operon?

Accepted Answer

A)  The transcript will be produced and normal in length
B)  The transcript will be produced, but shorter than normal
C)  The transcript will be produced, but longer than normal
D)  The transcript will produced, but will contain a deletion
E)   The transcript will not be produced C

Question 16

Repressor proteins are responsible for negative transcriptional regulation.

Accepted Answer

A) True 
 B)False

Question 17

A deletion in an operon removes the promoter. How will that affect the transcript that is produced from the operon?

Accepted Answer

A)  The transcript will be produced and normal in length
B)  The transcript will be produced, but shorter than normal
C)  The transcript will be produced, but longer than normal
D)  The transcript will produced, but will contain a deletion
E)   The transcript will not be produced

Question 18

In Jacob, Monod, and Pardee's experiment, what would have been the conclusion if all four tubes produced a yellow color when b-ONPG was added?

Accepted Answer

A)  Expression of the lac operon is constitutive whether lacI is functional or not
B)  LacI provides the binding site for the repressor
C)  LacI encodes a diffusible repressor
D)  The researcher added too much b-ONPG

Question 19

A mutation in the lacI gene prevents the gene product from binding allolactose. What will the expression level of the operon be in the absence of lactose?

Accepted Answer

A)  no transcription
B)  postive regulation
C)  constitutively active
D)  low level of expression A

Question 20

For these questions, match the following to its appropriate letter. Use each letter only once.

Accepted Answer

The answer of For these questions, match the following to...

Exam 14: Gene Regulation in Bacteria

DNA that contains instructions for two or more structural genes produces monocistronic mRNA.

Correct Answer
verified

In Jacob, Monod, and Pardee's experiment, how many functional copies of lacI were there in the merozygote?

Correct Answer
verified

How many promoters are in an operon?

Correct Answer
verified

Correct Answer
verified

Operons that code for catabolic enzyme systems are typically regulated by repressors.

Correct Answer
verified

A riboswitch only affects translation of an operon.

Correct Answer
verified
False

In the lac operon, the operator is an example of a trans-effect genetic regulation.

Correct Answer
verified

Enzymes involved in metabolism are most likely regulated via _____.

Correct Answer
verified

The regulation of protein function is called ______.

Correct Answer
verified

In the lac operon, the CAP site is located next to the __. When both lactose and glucose are present, this leads to a rate of transcription that is _.

Correct Answer
verified

In the lac operon, the operator site is recognized by an activator protein.

Correct Answer
verified

What stem-loop conformations favor attenuation?

Correct Answer
verified

If CAP could not bind to its CAP site, then what would be the result? Assume lactose is present in each scenario.

Correct Answer
verified

What is the gene responsible for attenuation in the trp operon?

Correct Answer
verified

A deletion in an operon removes the terminator. How will that affect the transcript that is produced from the operon?

Correct Answer
verified
C

Repressor proteins are responsible for negative transcriptional regulation.

Correct Answer
verified

A deletion in an operon removes the promoter. How will that affect the transcript that is produced from the operon?

Correct Answer
verified

In Jacob, Monod, and Pardee's experiment, what would have been the conclusion if all four tubes produced a yellow color when b-ONPG was added?

Correct Answer
verified

A mutation in the lacI gene prevents the gene product from binding allolactose. What will the expression level of the operon be in the absence of lactose?

Correct Answer
verified
A

For these questions, match the following to its appropriate letter. Use each letter only once.

Correct Answer
verified

Exam 14: Gene Regulation in Bacteria

DNA that contains instructions for two or more structural genes produces monocistronic mRNA.

Correct AnswerverifiedShow Answer

In Jacob, Monod, and Pardee's experiment, how many functional copies of lacI were there in the merozygote?

Correct AnswerverifiedShow Answer

How many promoters are in an operon?

Correct AnswerverifiedShow Answer

Correct AnswerverifiedShow Answer

Operons that code for catabolic enzyme systems are typically regulated by repressors.

Correct AnswerverifiedShow Answer

A riboswitch only affects translation of an operon.

Correct AnswerverifiedFalse

In the lac operon, the operator is an example of a trans-effect genetic regulation.

Correct AnswerverifiedShow Answer

Enzymes involved in metabolism are most likely regulated via _____.

Correct AnswerverifiedShow Answer

The regulation of protein function is called ______.

Correct AnswerverifiedShow Answer

In the lac operon, the CAP site is located next to the ____________. When both lactose and glucose are present, this leads to a rate of transcription that is ___________.

Correct AnswerverifiedShow Answer

In the lac operon, the operator site is recognized by an activator protein.

Correct AnswerverifiedShow Answer

What stem-loop conformations favor attenuation?

Correct AnswerverifiedShow Answer

If CAP could not bind to its CAP site, then what would be the result? Assume lactose is present in each scenario.

Correct AnswerverifiedShow Answer

What is the gene responsible for attenuation in the trp operon?

Correct AnswerverifiedShow Answer

A deletion in an operon removes the terminator. How will that affect the transcript that is produced from the operon?

Correct AnswerverifiedC

Repressor proteins are responsible for negative transcriptional regulation.

Correct AnswerverifiedShow Answer

A deletion in an operon removes the promoter. How will that affect the transcript that is produced from the operon?

Correct AnswerverifiedShow Answer

In Jacob, Monod, and Pardee's experiment, what would have been the conclusion if all four tubes produced a yellow color when b-ONPG was added?

Correct AnswerverifiedShow Answer

A mutation in the lacI gene prevents the gene product from binding allolactose. What will the expression level of the operon be in the absence of lactose?

Correct AnswerverifiedA

For these questions, match the following to its appropriate letter. Use each letter only once.

Correct AnswerverifiedShow Answer

Correct Answer
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False

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Correct Answer
verified

In the lac operon, the CAP site is located next to the __. When both lactose and glucose are present, this leads to a rate of transcription that is _.

Correct Answer
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Correct Answer
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C

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A

Correct Answer
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