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The elongation and termination phases of eukaryotic transcription


A) require several protein factors
B) are better understood than the initiation phase
C) are not subject to control mechanisms, only the initiation phase
D) proceed at a constant rate and always stop at the same termination sequence

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How do the core enzyme and the holoenzyme of RNA polymerase differ in


A) The holoenzyme includes the sigma (s) subunit, the core enzyme does not.
B) The core enzyme includes the sigma (s) subunit, the holoenzyme does not.
C) The holoenzyme transcribes from an RNA template, the core enzyme from a DNA template.
D) The core enzyme transcribes from an RNA template, the holoenzyme from a DNA template.
E) coli?

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Which of following statements describes a difference between replication of DNA and transcription of RNA?


A) Nucleoside triphosphates are the precursors for replication, but nucleoside diphosphates are used for transcription.
B) Both strands of DNA are copied in replication, but usually only one is copied in transcription.
C) Base pairing is used to copy the sequence in replication, but not in transcription.
D) The chain grows from the 5' to the 3' end in replication, but 3' to 5' in transcription.

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Which of the following is not a property of the sigma (s) subunit?


A) It tells the RNA Polymerase where to sit down.
B) It helps point the RNA Pol in the proper direction.
C) It causes the RNA Pol to bind tightly to the DNA.
D) It stays with the RNA Pol throughout synthesis.

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The enzyme principally responsible for RNA synthesis in Escherichia coli


A) is a multisubunit enzyme
B) consists of a single polypeptide chain
C) requires Mn2+ for activity
D) requires a DNA primer

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Which of the following offers the best description of a Pribnow box?


A) A promoter consensus sequence located at approximately -35.
B) A promoter consensus sequence located at approximately -10.
C) A sequence forming a hairpin loop signaling the termination of transcription.
D) A sequence immediately surrounding the start site of transcription.

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Which of the following is true about riboswitches:


A) They have a molecule sensing domain called an aptamer
B) There are known pathogens that use riboswitches as part of their mechanism
C) They respond to specific molecules and control translation in a way that is often similar to transcription attenuation
D) They contain a sensing domain and a decision making domain
E) All of these

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Which of the following is not true?


A) The mechanism of activation of eukaryotic genes involves addition and removal of phosphate residues from some of the transcription factors.
B) In plants, there are 5 RNA polymerases.
C) RNA Pol IV is the primary RNA synthesizer in plants
D) Of the RNA Polymerases in eukaryotes, Pol II is the most extensively studied.

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Which of the following is not a structural motif encountered in DNA-binding proteins?


A) helix-turn-helix
B) leucine zipper
C) zinc finger
D) b-barrel

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The major difference between RNA initiation in eukaryotes and prokaryotes is the number of factors involved in the process.

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Which of the following RNAs is noted for having a "cloverleaf" structure?


A) mRNA
B) rRNA
C) tRNA
D) All of these

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How do enhancers differ from promoters?


A) Enhancers do not bind RNA polymerase.
B) Enhancers include the UP element.
C) Enhancers bind the sigma (s) factor.
D) There is no difference; these terms are synonymous.

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Which of the following is not part of the core promoter?


A) the transcription start site
B) the Pribnow box (-10 region)
C) the -35 region
D) the UP element

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