Question 1

In precipitation tests,the antigen

Accepted Answer

A)   -antibody complex settles to the bottom of the tube.
B)   is a whole cell.
C)   is an insoluble molecule.
D)   is a soluble molecule.

Question 2

Soluble antigens are detected in what type of test?

Accepted Answer

A)   Specificity
B)   Cross-reactions
C)   Agglutination
D)   Precipitation
E)   Sensitivity

Question 3

If you were going to identify microorganisms within a specimen containing large numbers of organisms (seawater,normal microbiota,etc.) ,you would have to

Accepted Answer

A)   use a wide variety of microbiological media to identify all of the different species of microorganisms.
B)   use a wide variety of antibodies to react with the antigens.
C)   use different kinds of serological tests.
D)   amplify gene sequences within the sample to identify each of the different organisms.

Question 4

The dyes fluorescein and rhodamine

Accepted Answer

A)   can be used to label antibodies in immunofluorescence tests.
B)   emit visible light in response to ultraviolet radiation.
C)   are observed using fluorescence microscopy.
D)   are used to identify pathogens of chlamydiosis, Legionnaires'disease, and others.
E)   All of the choices are correct.

Question 5

The difference between presumptive and confirmatory data in specimen identification is that

Accepted Answer

A)   presumptive data affords a preliminary identification such as the genus, and confirmatory data identifies the species of the organism.
B)   presumptive data is an educated guess whereas confirmatory data identifies the specific genus and species of the organism.
C)   presumptive data is more accurate than confirmatory data.
D)   presumptive data identifies the species, and confirmatory data identifies the genus of the organism.

Question 6

Depending on the organism,direct examination of a specimen is often the most rapid diagnostic tool used in the clinical microbiology lab.You have a sample of pus from a patient's penis.What will be the easiest test to confirm that the patient has gonorrhea?

Accepted Answer

A)   Agglutination reaction
B)   Gram stain
C)   Microarray
D)   ELISA

Question 7

Which of these tests is an in vivo test?

Accepted Answer

A)   Tuberculin test
B)   Western Blot
C)   Agglutination test
D)   PCR

Question 8

The indirect ELISA test is commonly used to diagnose infections caused by all of the following except ______.

Accepted Answer

A)   hepatitis A virus
B)   rubella virus
C)   hepatitis C virus
D)   HIV
E)   Helicobacter

Question 9

Syphilis can be diagnosed by the

Accepted Answer

A)   Widal test.
B)   antistreptolysin O test.
C)   Weil-Felix reaction.
D)   latex agglutination test.
E)   rapid plasma reagin test.

Question 10

A positive direct ELISA result requires

Accepted Answer

A)   one known antibody, one unknown antibody, and an unknown antigen.
B)   one known antibody, one unknown antibody, and a known antigen.
C)   two known antibodies and one known antigen.
D)   two known antibodies and one unknown antigen.

Question 11

Antibiotics are often added to biochemical media to restrict the growth to only those organisms that are being looked for in the specimens.

Accepted Answer

A) True 
 B)False

Question 12

Both pulse-field gel electrophoresis and Western blotting utilize electrophoresis in their procedures.When comparing the two procedures,which statement is true?

Accepted Answer

A)   Pulse-field gel electrophoresis is used for genetic analysis.
B)   Pulse-field gel electrophoresis separates DNA fragments while Western blotting separates proteins.
C)   The presence of antibodies in a patient specimen can be identified in the Western Blot.
D)   Pulse-field gel electrophoresis is excellent for identifying minute genetic differences among strains of one species of an organism.
E)   All of the statements are true.

Question 13

The difference between direct and indirect immunofluorescence testing is that

Accepted Answer

A)   Direct testing adds antibodies directly to antigens, whereas indirect testing cultures the organisms first and adds fluorescent tags to the patient sample.
B)   Direct testing determines the identity of an unknown antibody in a patient sample, whereas indirect testing determines whether the patient is infected with a specific antigen.
C)   Indirect testing determines the identity of an unknown antigen in a patient sample, whereas direct testing determines whether the patient has been exposed to an antigen as evidenced by the presence of antibodies in their sera.
D)  Direct testing determines the identity of an unknown antigen in a patient sample, whereas indirect testing determines whether the patient has been exposed to an antigen as evidenced by the presence of antibodies in their sera.

Question 14

In vivo testing is useful for diagnosing ______.

Accepted Answer

A)   hepatitis A
B)   HIV
C)   tuberculosis
D)   whooping cough
E)   rubella

Question 15

Dr.John Doe,a research microbiologist for a university medical center,has been accused of sending Bacillus anthracis,the causative agent of anthrax,through the mail to the President.You,as a public health official with the CDC,have to determine if it is the same strain as the two strains of
A. Gram staining
B. Serological testing using agglutination
B. anthracis found cultured in his lab. Which method of identification would you use to compare these three bacterial samples?
C. Biochemical test identification
D. Pulse-field gel electrophoresis

Accepted Answer

A.Gram staining
B.Serological ...

Question 16

Staphylococcus aureus can be identified by mixing the organism with specific antibody and visually recognizing a clumping reaction between the antigen and the antibody.This is a(n) _______ test.

Accepted Answer

A)   microarray
B)   ELISA
C)   agglutination
D)   biochemical

Question 17

The property of a test to detect even small amounts of antibodies or antigens that are test targets is ______.

Accepted Answer

A)   cross-reaction
B)   agglutination
C)   precipitation
D)   specificity
E)   sensitivity

Question 18

Direct antigen testing differs from direct fluorescence testing in that the results

Accepted Answer

A)   require an electron microscope.
B)   are less accurate.
C)   are more accurate.
D)   require specialized equipment.
E)   are read with the naked eye.

Question 19

Alkaline phosphatase is an enzyme used in _____ tests.

Accepted Answer

A)   indirect fluorescent antibody
B)   ELISA
C)   Western blot
D)   direct fluorescent antibody

Question 20

You place an inoculum of your bacterium on a glass slide and add a drop of hydrogen peroxide reagent.Promptly you see bubbles appear,indicating the presence of a particular enzyme that the bacterium produces.What type of identification test does this exemplify?

Accepted Answer

A)   Precipitation test
B)   Immunochromatography
C)   Microarray
D)   Biochemical test

Exam 17: Diagnosing Infections

Both pulse-field gel electrophoresis and Western blotting utilize electrophoresis in their procedures.When comparing the two procedures,which statement is true?

Correct Answer
verified

The property of a test to detect even small amounts of antibodies or antigens that are test targets is ______.

Correct Answer
verified

Correct Answer
verified
A.Gram staining
B.Serological ...
View Answer

In vivo testing is useful for diagnosing ______.

Correct Answer
verified

A positive direct ELISA result requires

Correct Answer
verified

You place an inoculum of your bacterium on a glass slide and add a drop of hydrogen peroxide reagent.Promptly you see bubbles appear,indicating the presence of a particular enzyme that the bacterium produces.What type of identification test does this exemplify?

Correct Answer
verified

The dyes fluorescein and rhodamine

Correct Answer
verified

The indirect ELISA test is commonly used to diagnose infections caused by all of the following except ______.

Correct Answer
verified

Direct antigen testing differs from direct fluorescence testing in that the results

Correct Answer
verified

The difference between direct and indirect immunofluorescence testing is that

Correct Answer
verified

Antibiotics are often added to biochemical media to restrict the growth to only those organisms that are being looked for in the specimens.

Correct Answer
verified

Depending on the organism,direct examination of a specimen is often the most rapid diagnostic tool used in the clinical microbiology lab.You have a sample of pus from a patient's penis.What will be the easiest test to confirm that the patient has gonorrhea?

Correct Answer
verified

The difference between presumptive and confirmatory data in specimen identification is that

Correct Answer
verified

In precipitation tests,the antigen

Correct Answer
verified

If you were going to identify microorganisms within a specimen containing large numbers of organisms (seawater,normal microbiota,etc.) ,you would have to

Correct Answer
verified

Staphylococcus aureus can be identified by mixing the organism with specific antibody and visually recognizing a clumping reaction between the antigen and the antibody.This is a(n) _______ test.

Correct Answer
verified

Soluble antigens are detected in what type of test?

Correct Answer
verified

Which of these tests is an in vivo test?

Correct Answer
verified

Alkaline phosphatase is an enzyme used in _____ tests.

Correct Answer
verified

Syphilis can be diagnosed by the

Correct Answer
verified

Exam 17: Diagnosing Infections

Both pulse-field gel electrophoresis and Western blotting utilize electrophoresis in their procedures.When comparing the two procedures,which statement is true?

Correct AnswerverifiedShow Answer

The property of a test to detect even small amounts of antibodies or antigens that are test targets is ______.

Correct AnswerverifiedShow Answer

Correct AnswerverifiedA.Gram stainingB.Serological ...View AnswerShow Answer

In vivo testing is useful for diagnosing ______.

Correct AnswerverifiedShow Answer

A positive direct ELISA result requires

Correct AnswerverifiedShow Answer

You place an inoculum of your bacterium on a glass slide and add a drop of hydrogen peroxide reagent.Promptly you see bubbles appear,indicating the presence of a particular enzyme that the bacterium produces.What type of identification test does this exemplify?

Correct AnswerverifiedShow Answer

The dyes fluorescein and rhodamine

Correct AnswerverifiedShow Answer

The indirect ELISA test is commonly used to diagnose infections caused by all of the following except ______.

Correct AnswerverifiedShow Answer

Direct antigen testing differs from direct fluorescence testing in that the results

Correct AnswerverifiedShow Answer

The difference between direct and indirect immunofluorescence testing is that

Correct AnswerverifiedShow Answer

Antibiotics are often added to biochemical media to restrict the growth to only those organisms that are being looked for in the specimens.

Correct AnswerverifiedShow Answer

Depending on the organism,direct examination of a specimen is often the most rapid diagnostic tool used in the clinical microbiology lab.You have a sample of pus from a patient's penis.What will be the easiest test to confirm that the patient has gonorrhea?

Correct AnswerverifiedShow Answer

The difference between presumptive and confirmatory data in specimen identification is that

Correct AnswerverifiedShow Answer

In precipitation tests,the antigen

Correct AnswerverifiedShow Answer

If you were going to identify microorganisms within a specimen containing large numbers of organisms (seawater,normal microbiota,etc.) ,you would have to

Correct AnswerverifiedShow Answer

Staphylococcus aureus can be identified by mixing the organism with specific antibody and visually recognizing a clumping reaction between the antigen and the antibody.This is a(n) _______ test.

Correct AnswerverifiedShow Answer

Soluble antigens are detected in what type of test?

Correct AnswerverifiedShow Answer

Which of these tests is an in vivo test?

Correct AnswerverifiedShow Answer

Alkaline phosphatase is an enzyme used in _____ tests.

Correct AnswerverifiedShow Answer

Syphilis can be diagnosed by the

Correct AnswerverifiedShow Answer

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified
A.Gram staining
B.Serological ...
View Answer

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified