Question 1

Genetic engineering of plants has so far produced

Accepted Answer

A)   pest resistant plants.
B)   plants that are herbicide resistant.
C)   plants with increased nutritional value.
D)   All of the choices are correct.

Question 2

A DNA microarray contains oligonucleotides that contain a label.

Accepted Answer

A) True 
 B)False

Question 3

Which of the following genera has proved useful for manipulating plant cells?

Accepted Answer

A)   Escherichia
B)   Bacillus
C)   Pseudomonas
D)   Agrobacterium

Question 4

PCR is particularly useful in

Accepted Answer

A)   detecting viable yet non-culturable organisms.
B)   assessing impure (multiple types of bacteria present)  samples.
C)   dealing with very small numbers of bacteria.
D)   relatively quickly producing results.
E)   All of the choices are correct.

Question 5

Agarose gel electrophoresis may be considered as a partial purification technique.

Accepted Answer

A) True 
 B)False

Question 6

A common way to identify the

Accepted Answer

A)   vector.
B)   probe.
C)   host.
D)   plasmid.
E)   coli that carries the desired recombinant DNA is by using a

Question 7

The current cost of sequencing a human genome is about

Accepted Answer

A)   $ 10,000
B)   $ 250,000
C)   $ 1,000,000
D)   $ 4,000,000 D

Question 8

An ideal vector

Accepted Answer

A)   may be a plasmid or bacteriophage.
B)   has a restriction enzyme recognition site.
C)   contains an origin of replication.
D)   contains a selectable marker.
E)   All of the choices are correct.

Question 9

The molecule(s)  that act as molecular glue to bind DNA fragments together is/are

Accepted Answer

A)   DNAse.
B)   DNA ligase.
C)   ligandase.
D)   polymerase.
E)   DNAse AND ligandase. B

Question 10

The polymerase chain reaction is used to

Accepted Answer

A)   amplify certain sections of DNA.
B)   amplify mRNA.
C)   produce proteins.
D)   produce long polymers of carbohydrates to be used in electrophoresis.

Question 11

The size of the amplified DNA fragment generated during PCR is determined by

Accepted Answer

A)   how many cycles are performed.
B)   the size of the template DNA.
C)   the location to which the primers anneal.
D)   how much Taq is used.

Question 12

A danger in using E. coli in cloning is that

Accepted Answer

A)  E. coli could cause disease.
B)   the human cells may reject the insertion.
C)   the exons may invert the introns.
D)   the outer membrane is poisonous to humans.

Question 13

Starting with a single piece of dsDNA, after 3 PCR cycles there are

Accepted Answer

A)   2 additional pieces of dsDNA.
B)   4 additional pieces of dsDNA.
C)   8 additional pieces of dsDNA.
D)   16 additional pieces of dsDNA.

Question 14

The entire set of cloned fragments of the complete human genome is termed a

Accepted Answer

A)   book of genes.
B)   recombinant gene.
C)   DNA library.
D)   restructured genome.

Question 15

Double-stranded DNA will separate into two strands when exposed to

Accepted Answer

A)   high temperature.
B)   high pH.
C)   low temperature.
D)   low salt.
E)   high temperature AND high pH.

Question 16

Mitochondrial DNA (mtDNA)  in humans

Accepted Answer

A)   is always donated to the offspring from both parents
B)   can be used to identify related people
C)   can be isolated only from intact embryos
D)   can be used to establish paternity

Question 17

When using lacZ containing vectors, colonies containing intact vector turn blue.

Accepted Answer

A) True 
 B)False

Question 18

When a vector that employs the lacZ gene as a second marker is used in a cloning experiment, bacteria that harbor the recombinant DNA will give rise to

Accepted Answer

A)   red colonies.
B)   white colonies.
C)   blue colonies.
D)   All of the choices are correct. B

Question 19

The agarose used in electrophoresis

Accepted Answer

A)   interacts electrically with the DNA.
B)   chemically binds to the DNA.
C)   acts as a sieve.
D)   selectively sorts recombinant DNA from host DNA.

Question 20

Vectors must have at least one restriction enzyme recognition site.

Accepted Answer

A) True 
 B)False

Exam 9: Biotechnology and Recombinant Dna

Genetic engineering of plants has so far produced

Correct AnswerverifiedShow Answer

A DNA microarray contains oligonucleotides that contain a label.

Correct AnswerverifiedShow Answer

Which of the following genera has proved useful for manipulating plant cells?

Correct AnswerverifiedShow Answer

PCR is particularly useful in

Correct AnswerverifiedShow Answer

Agarose gel electrophoresis may be considered as a partial purification technique.

Correct AnswerverifiedShow Answer

A common way to identify the

Correct AnswerverifiedShow Answer

The current cost of sequencing a human genome is about

Correct AnswerverifiedD

An ideal vector

Correct AnswerverifiedShow Answer

The molecule(s) that act as molecular glue to bind DNA fragments together is/are

Correct AnswerverifiedB

The polymerase chain reaction is used to

Correct AnswerverifiedShow Answer

The size of the amplified DNA fragment generated during PCR is determined by

Correct AnswerverifiedShow Answer

A danger in using E. coli in cloning is that

Correct AnswerverifiedShow Answer

Starting with a single piece of dsDNA, after 3 PCR cycles there are

Correct AnswerverifiedShow Answer

The entire set of cloned fragments of the complete human genome is termed a

Correct AnswerverifiedShow Answer

Double-stranded DNA will separate into two strands when exposed to

Correct AnswerverifiedShow Answer

Mitochondrial DNA (mtDNA) in humans

Correct AnswerverifiedShow Answer

When using lacZ containing vectors, colonies containing intact vector turn blue.

Correct AnswerverifiedShow Answer

When a vector that employs the lacZ gene as a second marker is used in a cloning experiment, bacteria that harbor the recombinant DNA will give rise to

Correct AnswerverifiedB

The agarose used in electrophoresis

Correct AnswerverifiedShow Answer

Vectors must have at least one restriction enzyme recognition site.

Correct AnswerverifiedShow Answer

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D

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B

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B

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