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One of the first chemicals used by Lister to prevent surgical infections was


A) alcohol.
B) iodine.
C) carbolic acid.
D) mercury.

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Heat treatment is an effective method for sterilization or disinfection of all materials.

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Chlorine


A) readily reacts with organics to produce potentially carcinogenic trihalomethanes.
B) is an effective, inexpensive, disinfectant able to destroy all types of microorganisms.
C) is unaffected by the presence of organic material.
D) is ineffective when diluted.
E) readily reacts with organics to produce potentially carcinogenic trihalomethanes AND is an effective, inexpensive, disinfectant able to destroy all types of microorganisms.

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Commercial canning processes


A) are geared to destroy Clostridium botulinum spores.
B) kill all endospores.
C) are especially needed on low acid foods.
D) are 12D processes (designed to kill 1012 endospores) .
E) are geared to destroy Clostridium botulinum spores, are especially needed on low acid foods, AND are 12D (designed to kill 1012 endospores) processes.

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Hexachlorophene has been particularly effective against


A) Staphylococcus aureus.
B) Micrococcus aureus.
C) Escherichia coli.
D) Enterobacter aerogenes.

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Which of the following methods sterilize the materials?


A) Pasteurization
B) High-temperature-short-time pasteurization (HTST)
C) Ultrahigh-temperature (UHT) method
D) None of these are sterilization methods

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Generally, heavy metals, except silver, have been proven to be too toxic for use on human tissue and are no longer used medically.

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Cold and freezing are very effective in killing bacteria.

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Gamma irradiation


A) has not been approved for use on food.
B) can be used to either sterilize or pasteurize, depending on the dose of radiation.
C) leaves some radioactive particles in the treated substance.
D) usually kills by disrupting cell membranes.

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If a process kills 90% of the organisms per minute, how many minutes would it take to kill all organisms when starting with 100,000 organisms?


A) 1 minute
B) 2 minutes
C) 3 minutes
D) 6 minutes

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Dry heat takes a much shorter time to sterilize material than wet heat.

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Upon heat treatment, bacteria die at a constant proportion.

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Lister developed his ideas on prevention of infection during medical procedures after studying the work of


A) Koch.
B) Pasteur.
C) Jenner.
D) Fleming.

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Moist heat kills microorganisms by


A) irreversible coagulation of proteins.
B) destruction of carbohydrates in the cell wall.
C) denaturation of nucleic acids.
D) dissolving the capsule.

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Which is true of iodine?


A) It does not readily kill endospores.
B) It may be used as an antiseptic or as a disinfectant.
C) It is important to use it at the recommended dilution.
D) It is usually found as tinctures or iodophors.
E) All of the choices are true.

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Boiling is very effective at removing most common waterborne pathogens.

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Which of the following is true of hydrogen peroxide?


A) It is a sterilant for inanimate objects and is quickly inactivated on living tissue.
B) It leaves a toxic residue.
C) It is broken down by catalase into water and oxygen.
D) It is even more effective when used in combination with peracetic acid.
E) It is a sterilant for inanimate objects and is quickly inactivated on living tissue; it is broken down by catalase into water and oxygen AND it is even more effective when used in combination with peracetic acid.

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Typical conditions used for sterilization are


A) 100°C for 10 minutes.
B) 121°C at 15 psi for 15 minutes.
C) 80°C for 15 minutes.
D) 72°C for 15 seconds.

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A suffix used to describe a killing action would be


A) -static.
B) -cidal.
C) -cillin.
D) -tion.

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Which of the following organisms are resistant to destruction by typical control methods?


A) endospores of Bacillus and Clostridium
B) Pseudomonas
C) naked viruses
D) Mycobacterium spp.
E) All of the choices are correct.

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