Question 1

Place the following steps in order for the cloning of a gene.1.DNA ligase seals foreign DNA into a plasmid.2.A restriction enzyme is used to cleave DNA.3.The bacterium makes a product.4.Some of the bacterial cells take up a recombinant plasmid.5.The plasmid replicates on its own.

Accepted Answer

A)  2,1,4,5,3
B)  1,2,3,4,5
C)  3,1,2,4,5
D)  5,1,4,3,2
E)  1,3,2,4,5

Question 2

Which of the following is not a desirable trait for a genetically engineered plant?

Accepted Answer

A)  disease resistant
B)  salt sensitive
C)  improved yield
D)  modified wood pulp
E)  drought tolerant

Question 3

Which of the following statements about PCR is not true?

Accepted Answer

A)  PCR stands for "polymerizing copies of RNA.""
B)  PCR can create millions of copies of a segment of DNA in a test tube.
C)  PCR uses DNA polymerase.
D)  The amount of DNA doubles with each replication cycle.
E)  The target is repeatedly replicated.

Question 4

Mitochondria also contain DNA.

Accepted Answer

A) True 
 B)False

Question 5

One of the disadvantages of PCR is that it requires large amounts of starting material.

Accepted Answer

A) True 
 B)False

Question 6

Ex vivo gene therapy has been used to treat which of the following diseases?

Accepted Answer

A)  SCID
B)  cardiovascular disease
C)  Huntington disease
D)  endocrine disorder
E)  all of these

Question 7

What is the name of the enzyme that fits new complementary DNA nucleotides into the new strand?

Accepted Answer

A)  DNA polymerase
B)  DNA helicase
C)  DNA replicase
D)  DNA ligase
E)  DNA telomerase

Question 8

DNA replication is considered

Accepted Answer

A)  conservative.
B)  semiconservative.
C)  dispersive.
D)  relaxeD.
E)  stringent.

Question 9

Which of the following occurs during initiation of translation?

Accepted Answer

A)  The ribosome moves down one codon so that the A site is available to receive an incoming tRNA.
B)  The mRNA binds to the smaller of the two ribosomal subunits.
C)  The polypeptide lengthens one amino acid at a time.
D)  The ribosome dissociates into its two subunits and falls off the mRNA.
E)  One of the three stop codons is reached.

Question 10

Both DNA and RNA are

Accepted Answer

A)  found in the cytoplasm.
B)  transcribed.
C)  translated.
D)  found in the nucleus.
E)  considered the genetic material of the cell.

Question 11

In vivo gene therapy has been used to help patients suffering from cystic fibrosis.

Accepted Answer

A) True 
 B)False

Question 12

How many different amino acids are commonly found in proteins?

Accepted Answer

A)  5
B)  10
C)  20
D)  50
E)  over 100

Question 13

It only takes a matter of several hours to sequence 1000 base-pair sections of DNA.

Accepted Answer

A) True 
 B)False

Question 14

Which of the following is the primary goal of bioinformatics?

Accepted Answer

A)  to find significant patterns in the information gained from DNA sequencing and proteomics
B)  to input DNA and protein sequences into a computer
C)  to model the three-dimensional shape of cellular proteins
D)  to obtain the sequence of all the noncoding regions of the DNA
E)  to insert genetic material into human cells for the treatment of a disorder

Question 15

Which structure contains the anticodon?

Accepted Answer

A)  the mRNA
B)  the large ribosomal subunit
C)  the small ribosomal subunit
D)  the tRNA
E)  the rRNA

Question 16

Which of the following is a benefit gained from learning how to sequence DNA?

Accepted Answer

A)  identification of faulty genes
B)  development of treatments for diseases
C)  modification of crops to increase yields
D)  understanding of our evolutionary history
E)  all of these are benefits gained from DNA sequencing

Question 17

How is the recombinant product procured from transgenic bacteria? Transgenic goats?

Accepted Answer

A)  eat them,from growth media
B)  from growth media,drink milk
C)  eat them,eat them
D)  drink milk,drink milk
E)  from growth media,from growth media

Question 18

Which of the following is not true about transcription factors?

Accepted Answer

A)  Transcription factors are DNA-binding proteins.
B)  Transcription factors are considered external cell regulators.
C)  There are many different types of transcription factors.
D)  Specialization of cells is determined/influenced by which transcription factors are active in the cell.
E)  Genes code for transcription factors.

Question 19

Which of the following nucleotide sequences could give rise to the amino acid sequence alanine-asparatate-glycine? Assume that translation initiation has already occurred.

Accepted Answer

A)  GUUAAGAGG
B)  GCCGACGGC
C)  CACCGCCGA
D)  GAUGGUGCU
E)  UUUUAAUGG

Question 20

How much of your DNA is identical to the person who sits next to you in biology class?

Accepted Answer

A)  1%
B)  50%
C)  90%
D)  99%
E)  99.9%

Exam 22: DNA Biology and Technology

It only takes a matter of several hours to sequence 1000 base-pair sections of DNA.

Correct AnswerverifiedShow Answer

Which of the following statements about PCR is not true?

Correct AnswerverifiedShow Answer

What is the name of the enzyme that fits new complementary DNA nucleotides into the new strand?

Correct AnswerverifiedShow Answer

Both DNA and RNA are

Correct AnswerverifiedShow Answer

Which of the following is not a desirable trait for a genetically engineered plant?

Correct AnswerverifiedShow Answer

One of the disadvantages of PCR is that it requires large amounts of starting material.

Correct AnswerverifiedShow Answer

How is the recombinant product procured from transgenic bacteria? Transgenic goats?

Correct AnswerverifiedShow Answer

Which of the following occurs during initiation of translation?

Correct AnswerverifiedShow Answer

How much of your DNA is identical to the person who sits next to you in biology class?

Correct AnswerverifiedShow Answer

How many different amino acids are commonly found in proteins?

Correct AnswerverifiedShow Answer

Place the following steps in order for the cloning of a gene.1.DNA ligase seals foreign DNA into a plasmid.2.A restriction enzyme is used to cleave DNA.3.The bacterium makes a product.4.Some of the bacterial cells take up a recombinant plasmid.5.The plasmid replicates on its own.

Correct AnswerverifiedShow Answer

DNA replication is considered

Correct AnswerverifiedShow Answer

Which structure contains the anticodon?

Correct AnswerverifiedShow Answer

Which of the following is a benefit gained from learning how to sequence DNA?

Correct AnswerverifiedShow Answer

Which of the following nucleotide sequences could give rise to the amino acid sequence alanine-asparatate-glycine? Assume that translation initiation has already occurred.

Correct AnswerverifiedShow Answer

Mitochondria also contain DNA.

Correct AnswerverifiedShow Answer

Which of the following is not true about transcription factors?

Correct AnswerverifiedShow Answer

In vivo gene therapy has been used to help patients suffering from cystic fibrosis.

Correct AnswerverifiedShow Answer

Ex vivo gene therapy has been used to treat which of the following diseases?

Correct AnswerverifiedShow Answer

Which of the following is the primary goal of bioinformatics?

Correct AnswerverifiedShow Answer

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