Question 1

In E. coli, dnaA protein binds to the __________________.

Accepted Answer

origin of ...

Question 2

Why is DNA gyrase the target for several different antibiotics?

Accepted Answer

DNA gyrase is the name given to Type II ...

Question 3

How are single-stranded regions of DNA maintained?

Accepted Answer

Single-stranded bind...

Question 4

How is the processivity of DNA polymerase III accomplished?

Accepted Answer

The structure of the enzyme complex allows the reaction processivity. The enzyme is a large complex that forms a ring around the DNA polymer. A specific subunit leaves enough space for the newly assembled DNA to be formed properly, and acts as a sliding DNA clamp.

Question 5

Write the chemical reaction for the addition of the dNTP to a DNA strand at the 3` end.

Accepted Answer

The answer should indicate cle...

Question 6

Use the following to answer questions
Choose the correct answer from the list below. Not all of the answers will be used.
-DNA can serve as a(n)  _______________ to direct synthesis of the complementary strand of DNA or RNA.

Accepted Answer

A)  helicases
B)  Type I
C)  Type II
D)  G-rich strand
E)  telomeresF) topoisomerasesG) DNA polymeraseH) A-DNAI) trombone-slide modelJ) templateK) HollidayL) Okazaki

Question 7

Use the following to answer questions
Choose the correct answer from the list below. Not all of the answers will be used.
-The type of topoisomerase that does not require ATP is _______________.

Accepted Answer

A)  helicases
B)  Type I
C)  Type II
D)  G-rich strand
E)  telomeresF) topoisomerasesG) DNA polymeraseH) A-DNAI) trombone-slide modelJ) templateK) HollidayL) Okazaki

Question 8

Elongation of the DNA strand proceeds in the __________________ direction.

Accepted Answer

5` → 3`

Question 9

DNA clamps:

Accepted Answer

A)   are composed of β2 subunits.
B)   form a ring around the front of the fork of DNA.
C)   allow the polymerase to move along the DNA molecule without falling off.
D)   All of the above.
E)   None of the above. D

Question 10

Use the following to answer questions
Choose the correct answer from the list below. Not all of the answers will be used.
-The strands of DNA must be relaxed or unwound for replication by _______________.

Accepted Answer

A)  helicases
B)  Type I
C)  Type II
D)  G-rich strand
E)  telomeresF) topoisomerasesG) DNA polymeraseH) A-DNAI) trombone-slide modelJ) templateK) HollidayL) Okazaki

Question 11

Type ________________ topoisomerase introduces negative supercoils in DNA, while type ________________ topoisomerase relaxes supercoil structures.

Accepted Answer

The answer of Type ________________ topoisomerase introduces negative supercoils in...

Question 12

The dimeric β2 subunit of DNA polymerase III acts as a(n) __________________.

Accepted Answer

The answer of The dimeric β2 subunit of DNA polymerase...

Question 13

During DNA replication, the RNA primer pieces are removed by __________________.

Accepted Answer

The answer of During DNA replication, the RNA primer pieces...

Question 14

Why are enzymes, other than DNA polymerase, required for replication?

Accepted Answer

DNA is an extremely stable molecule with...

Question 15

What function does helicase perform during replication?

Accepted Answer

A)   It stabilizes the negative charge of the transition state.
B)   It utilizes ATP to power strand separation.
C)   It adds negative supercoils to DNA.
D)   A and C.
E)   None of the above.

Question 16

Proofreading by DNA polymerase increases accuracy by a factor of _________________ times.

Accepted Answer

The answer of Proofreading by DNA polymerase increases accuracy by...

Question 17

The term processivity:

Accepted Answer

A)   indicates the error rate for a polymerase.
B)   is the deletion of one or more bases in the DNA.
C)   is specifically the speed of replication.
D)   is the ability to catalyze many reactions without releasing substrate.
E)   A, B, and C.

Question 18

The free end of the eukaryotic chromosome is thought to form a unique DNA structure that allows for complete replication. This structure involves a G-rich repeating sequence and forms a:

Accepted Answer

A)   replication fork.
B)   large duplex loop.
C)   supercoiled loop.
D)   displacement loop
E)   None of the above.

Question 19

What is true about E. coli DNA polymerases?

Accepted Answer

A)   There are five structural classes.
B)   All have finger and thumb domains that wrap around the DNA.
C)   All catalyze the same reaction, which requires metal cofactors.
D)   B and C.
E)   A, B, and C.

Question 20

Use the following to answer questions
Choose the correct answer from the list below. Not all of the answers will be used.
-The _______________ describes the lengthening of the loop formed by the lagging strand during replication.

Accepted Answer

A)  helicases
B)  Type I
C)  Type II
D)  G-rich strand
E)  telomeresF) topoisomerasesG) DNA polymeraseH) A-DNAI) trombone-slide modelJ) templateK) HollidayL) Okazaki

Exam 34: DNA Replication

In E. coli, dnaA protein binds to the __________________.

Correct Answerverifiedorigin of ...View AnswerShow Answer

Why is DNA gyrase the target for several different antibiotics?

Correct AnswerverifiedDNA gyrase is the name given to Type II ...View AnswerShow Answer

How are single-stranded regions of DNA maintained?

Correct AnswerverifiedSingle-stranded bind...View AnswerShow Answer

How is the processivity of DNA polymerase III accomplished?

Correct AnswerverifiedThe structure of the enzyme complex allows the reaction processivity. The enzyme is a large complex that forms a ring around the DNA polymer. A specific subunit leaves enough space for the newly assembled DNA to be formed properly, and acts as a sliding DNA clamp.

Write the chemical reaction for the addition of the dNTP to a DNA strand at the 3` end.

Correct AnswerverifiedThe answer should indicate cle...View AnswerShow Answer

Use the following to answer questions Choose the correct answer from the list below. Not all of the answers will be used. -DNA can serve as a(n) _______________ to direct synthesis of the complementary strand of DNA or RNA.

Correct AnswerverifiedShow Answer

Use the following to answer questions Choose the correct answer from the list below. Not all of the answers will be used. -The type of topoisomerase that does not require ATP is _______________.

Correct AnswerverifiedShow Answer

Elongation of the DNA strand proceeds in the __________________ direction.

Correct Answerverified5` → 3`

DNA clamps:

Correct AnswerverifiedD

Use the following to answer questions Choose the correct answer from the list below. Not all of the answers will be used. -The strands of DNA must be relaxed or unwound for replication by _______________.

Correct AnswerverifiedShow Answer

Type ________________ topoisomerase introduces negative supercoils in DNA, while type ________________ topoisomerase relaxes supercoil structures.

Correct AnswerverifiedShow Answer

The dimeric β2 subunit of DNA polymerase III acts as a(n) __________________.

Correct AnswerverifiedShow Answer

During DNA replication, the RNA primer pieces are removed by __________________.

Correct AnswerverifiedShow Answer

Why are enzymes, other than DNA polymerase, required for replication?

Correct AnswerverifiedDNA is an extremely stable molecule with...View AnswerShow Answer

What function does helicase perform during replication?

Correct AnswerverifiedShow Answer

Proofreading by DNA polymerase increases accuracy by a factor of _________________ times.

Correct AnswerverifiedShow Answer

The term processivity:

Correct AnswerverifiedShow Answer

The free end of the eukaryotic chromosome is thought to form a unique DNA structure that allows for complete replication. This structure involves a G-rich repeating sequence and forms a:

Correct AnswerverifiedShow Answer

What is true about E. coli DNA polymerases?

Correct AnswerverifiedShow Answer

Use the following to answer questions Choose the correct answer from the list below. Not all of the answers will be used. -The _______________ describes the lengthening of the loop formed by the lagging strand during replication.

Correct AnswerverifiedShow Answer

Correct Answer
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origin of ...
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DNA gyrase is the name given to Type II ...
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Correct Answer
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Single-stranded bind...
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Correct Answer
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The structure of the enzyme complex allows the reaction processivity. The enzyme is a large complex that forms a ring around the DNA polymer. A specific subunit leaves enough space for the newly assembled DNA to be formed properly, and acts as a sliding DNA clamp.

Correct Answer
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The answer should indicate cle...
View Answer

Correct Answer
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Correct Answer
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Correct Answer
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5` → 3`

Correct Answer
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D

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Type topoisomerase introduces negative supercoils in DNA, while type topoisomerase relaxes supercoil structures.

Correct Answer
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Correct Answer
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Correct Answer
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Correct Answer
verified
DNA is an extremely stable molecule with...
View Answer

Correct Answer
verified

Correct Answer
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Correct Answer
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Correct Answer
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Correct Answer
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Correct Answer
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