Question 1

How are breaks sealed in discontinuous DNA formed during replication?

Accepted Answer

DNA ligase is the enzyme that seals the ...

Question 2

How does the trombone-slide mechanism work to coordinate replication?

Accepted Answer

The mechanism allows a directi...

Question 3

Choose the correct answer from the list below. Not all of the answers will be used.
-The strands of DNA must be relaxed or unwound for replication by _______________.

Accepted Answer

A)  helicases
B)  Type I
C)  Type II
D)  G-rich strand
E)  telomeresF) topoisomerasesG) DNA polymeraseH) A-DNAI) trombone-slide modelJ) templateK) HollidayL) Okazaki

Question 4

DNA clamps:

Accepted Answer

A)   are composed of β2 subunits.
B)   form a ring around the front of the fork of DNA.
C)   allow the polymerase to move along the DNA molecule without falling off.
D)   All of the above.
E)   None of the above.

Question 5

Choose the correct answer from the list below. Not all of the answers will be used.
-DNA can serve as a(n)  _______________ to direct synthesis of the complementary strand of DNA or RNA.

Accepted Answer

A)  helicases
B)  Type I
C)  Type II
D)  G-rich strand
E)  telomeresF) topoisomerasesG) DNA polymeraseH) A-DNAI) trombone-slide modelJ) templateK) HollidayL) Okazaki

Question 6

During DNA replication, the RNA primer pieces are removed by __________________.

Accepted Answer

The answer of During DNA replication, the RNA primer pieces...

Question 7

How is the processivity of DNA polymerase III accomplished?

Accepted Answer

The structure of the enzyme complex allo...

Question 8

Replication moves from the:

Accepted Answer

A)   5` → 3` direction.
B)   3` → 5` direction.
C)   5` → 5` direction.
D)   3` → 3` direction.
E)   None of the above.

Question 9

coli, dnaA protein binds to the __________________.

Accepted Answer

origin of ...

Question 10

What is DNA gyrase?

Accepted Answer

A)   a bacterial topoisomerase II
B)   a bacterial topoisomerase I
C)   a bacterial helicase
D)   All of the above.
E)   None of the above.

Question 11

The free end of the eukaryotic chromosome is thought to form a unique DNA structure that allows for complete replication.This structure involves a G-rich repeating sequence and forms a:

Accepted Answer

A)   replication fork.
B)   large duplex loop.
C)   supercoiled loop.
D)   displacement loop
E)   None of the above.

Question 12

The Klenow fragment:

Accepted Answer

A)   has primase activity.
B)   is an E. coli DNA polymerase fragment.
C)   has exonuclease activity.
D)   A and B.
E)   B and C.

Question 13

Elongation of the DNA strand proceeds in the __________________ direction.

Accepted Answer

The answer of Elongation of the DNA strand proceeds in...

Question 14

Choose the correct answer from the list below. Not all of the answers will be used.
-The type of topoisomerase that does not require ATP is _______________.

Accepted Answer

A)  helicases
B)  Type I
C)  Type II
D)  G-rich strand
E)  telomeresF) topoisomerasesG) DNA polymeraseH) A-DNAI) trombone-slide modelJ) templateK) HollidayL) Okazaki

Question 15

How can the leading and lagging strands be synthesized in a coordinated fashion?

Accepted Answer

A)   Specific enzymes control the size of the DNA opening.
B)   Lagging-strand binding proteins inhibit leading-strand replication if the strands become disproportionate in size.
C)   Polymerase III is a dimeric holoenzyme, and the looped lagging strand allows the enzyme to proceed in the same direction with each strand.
D)   All of the above.
E)   None of the above.

Question 16

Write the chemical reaction for the addition of the dNTP to a DNA strand at the 3` end.

Accepted Answer

The answer should indicate cle...

Question 17

What function does helicase perform during replication?

Accepted Answer

A)   It stabilizes the negative charge of the transition state.
B)   It utilizes ATP to power strand separation.
C)   It adds negative supercoils to DNA.
D)   A and C.
E)   None of the above.

Question 18

Choose the correct answer from the list below. Not all of the answers will be used.
-The _______________ describes the lengthening of the loop formed by the lagging strand during replication.

Accepted Answer

A)  helicases
B)  Type I
C)  Type II
D)  G-rich strand
E)  telomeresF) topoisomerasesG) DNA polymeraseH) A-DNAI) trombone-slide modelJ) templateK) HollidayL) Okazaki

Question 19

What is the difference between Type I and Type II topoisomerase with respect to the thermodynamics of the reactions catalyzed?

Accepted Answer

Type I catalyzes the relaxatio...

Question 20

Choose the correct answer from the list below. Not all of the answers will be used.
-_______________ catalyzes the addition of deoxyribonucleotides to DNA.

Accepted Answer

A)  helicases
B)  Type I
C)  Type II
D)  G-rich strand
E)  telomeresF) topoisomerasesG) DNA polymeraseH) A-DNAI) trombone-slide modelJ) templateK) HollidayL) Okazaki

Exam 34: DNA Replication

Choose the correct answer from the list below. Not all of the answers will be used. -The strands of DNA must be relaxed or unwound for replication by _______________.

Correct AnswerverifiedShow Answer

Choose the correct answer from the list below. Not all of the answers will be used. -The _______________ describes the lengthening of the loop formed by the lagging strand during replication.

Correct AnswerverifiedShow Answer

What function does helicase perform during replication?

Correct AnswerverifiedShow Answer

During DNA replication, the RNA primer pieces are removed by __________________.

Correct AnswerverifiedShow Answer

DNA clamps:

Correct AnswerverifiedShow Answer

Choose the correct answer from the list below. Not all of the answers will be used. -The type of topoisomerase that does not require ATP is _______________.

Correct AnswerverifiedShow Answer

Replication moves from the:

Correct AnswerverifiedShow Answer

How is the processivity of DNA polymerase III accomplished?

Correct AnswerverifiedThe structure of the enzyme complex allo...View AnswerShow Answer

What is DNA gyrase?

Correct AnswerverifiedShow Answer

Elongation of the DNA strand proceeds in the __________________ direction.

Correct AnswerverifiedShow Answer

What is the difference between Type I and Type II topoisomerase with respect to the thermodynamics of the reactions catalyzed?

Correct AnswerverifiedType I catalyzes the relaxatio...View AnswerShow Answer

Write the chemical reaction for the addition of the dNTP to a DNA strand at the 3` end.

Correct AnswerverifiedThe answer should indicate cle...View AnswerShow Answer

How does the trombone-slide mechanism work to coordinate replication?

Correct AnswerverifiedThe mechanism allows a directi...View AnswerShow Answer

How are breaks sealed in discontinuous DNA formed during replication?

Correct AnswerverifiedDNA ligase is the enzyme that seals the ...View AnswerShow Answer

The free end of the eukaryotic chromosome is thought to form a unique DNA structure that allows for complete replication.This structure involves a G-rich repeating sequence and forms a:

Correct AnswerverifiedShow Answer

How can the leading and lagging strands be synthesized in a coordinated fashion?

Correct AnswerverifiedShow Answer

The Klenow fragment:

Correct AnswerverifiedShow Answer

Choose the correct answer from the list below. Not all of the answers will be used. -_______________ catalyzes the addition of deoxyribonucleotides to DNA.

Correct AnswerverifiedShow Answer

coli, dnaA protein binds to the __________________.

Correct Answerverifiedorigin of ...View AnswerShow Answer

Choose the correct answer from the list below. Not all of the answers will be used. -DNA can serve as a(n) _______________ to direct synthesis of the complementary strand of DNA or RNA.

Correct AnswerverifiedShow Answer

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The structure of the enzyme complex allo...
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Correct Answer
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Correct Answer
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Type I catalyzes the relaxatio...
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The answer should indicate cle...
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The mechanism allows a directi...
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DNA ligase is the enzyme that seals the ...
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Correct Answer
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Correct Answer
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Correct Answer
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origin of ...
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