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Describe the DNA helix proposed by Watson and Crick.

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The DNA model proposed by Watson and Cri...

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Choose the correct answer from the list below. Not all of the answers will be used. -The axis of a double helix can be twisted by a mechanism called _______________.


A) supercoiling
B) DNA
C) 5` → 3`
D) circular
E) stem loop
F) B-DNA
G) retroviruses
H) exon
I) Erwin Chargaff
J) histones
K) RNA
L) Rosalind Franklin
M) A-DNA
N) 3` → 5`

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What role does a nucleosome play in DNA structure?

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A nucleosome consists of approximately 2...

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The process of rehybridizing melted DNA is called:


A) denaturation.
B) Tm.
C) annealing.
D) dehybridization.
E) replication.

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Proteins that bind to DNA are rich the amino acids __________________ and __________________.

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How does a nucleotide differ from a nucleoside?


A) Nucleosides are found in DNA, whereas nucleotides are found in RNA.
B) Purines are only found in nucleotides.
C) Nucleosides contain only deoxyribose sugars.
D) A nucleotide is a nucleoside with a phosphate ester linked to the sugar .
E) None of the above.

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The __________________ effect stabilizes the structure of DNA, resulting in more polar surfaces being exposed to the aqueous media.

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Draw two nucleotides in DNA, showing the linkage between the sugars.

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The 3` oxygen of one nucleotide is linke...

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Why is there a major and a minor groove in DNA?

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The major and minor grooves arise becaus...

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What are topoisomers of DNA?

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Topoisomers, or topological isomers, are...

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The chemical forces that contribute to the stability of the DNA due to the base stacking present in the DNA helix are:


A) hydrogen bonds.
B) van der Waals interactions.
C) disulfide bonds.
D) C and C.
E) None of the above.

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Compare and contrast the three forms of DNA with respect to handedness and hydration state.

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The Watson and Crick model of DNA is known as B-DNA and is a right-handed helix. A-DNA is also a right-handed helix, but is a less hydrated form. Z-DNA is a left-handed helix characterized by a zigzag arrangement of the backbone phosphoryl groups.

Replication takes place in which manner?


A) conservative
B) random
C) semiconservative
D) N-linked
E) None of the above.

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How many different sequence possibilities are there for a DNA polymer that is 10 bases long?


A) 262,13232
B) 320
C) 32,000
D) 1,048,576
E) 100,000

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The shorthand notation pApCpG denotes that:


A) a phosphate is attached to the 5` of the adenosine nucleotide unit.
B) a phosphate is attached to the 3` of the guanine nucleotide unit.
C) the cytosine nucleotide has a free hydroxyl group.
D) A and C.
E) All of the above.

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Choose the correct answer from the list below. Not all of the answers will be used. -The stable genetic information passed on from one generation to the next is _______________.


A) supercoiling
B) DNA
C) 5` → 3`
D) circular
E) stem loop
F) B-DNA
G) retroviruses
H) exon
I) Erwin Chargaff
J) histones
K) RNA
L) Rosalind Franklin
M) A-DNA
N) 3` → 5`

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A nitrogen containing an aromatic base attached to a ribose is a __________________.

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The form of DNA that is the narrowestbut having the longest pitch per turn of helix is the:


A) A form
B) B form
C) C form
D) Z form
E) None of the above.

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D

Briefly describe the Meselson and Stahl experiment that indicated that DNA replication is semiconservative

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Meselson and Stahl grew bacteria in the presence of 15N, or "heavy" nitrogen. They rapidly replaced the 15N media with 14N, or "light" nitrogen. DNA was extracted at various intervals during the growth of the bacteria, representing different generations. Meselson and Stahl examined the DNA using density-gradient centrifugation and observed that no "heavy" DNA was present in the first generation, but that the DNA was intermediate between light and heavy. After the second generation, there were equal amounts of intermediate and light. This confirmed that one strand of the parent DNA is present in each daughter strand of replicated DNA.

The difference in RNA bases compared to DNA bases is:


A) RNA contains A instead of T.
B) RNA contains U instead of G.
C) RNA contains U instead of T.
D) RNA contains A instead of U.
E) None of the above.

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